技术文章
| Fluorophore | Peak Excitation λ (nm) | Peak Emission λ (nm) | Intersection λ (nm) | Type | Center Wavelength (nm) | Optical Density OD | Bandwith (nm) | Minimum Transmission (%) |
| 1,8-ANS (BSA) | 374.9 | 477 | 422.9 | Excitation | 377 | 6 | 50 | 85 |
| Emission | 480 | 3 | 10 | 45 | ||||
| 2-dodecylresorufin (lipid) | 581.9 | 595 | 589.5 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 593 | 6 | 40 | 90 | ||||
| 5-(and-6)-carboxy-2',7'-dichlorofluorescein (pH 9.0) | 504 | 524.9 | 513.8 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| 5-carboxytetramethylrhodamine [5-TAMRA] (MeOH) | 542.9 | 567 | 554.4 | Excitation | 543 | 6 | 22 | 90 |
| Emission | 568 | 3 | 10 | 45 | ||||
| 5-chloromethylfluorescein (pH 7.2) | 490.9 | 517 | 501.8 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| 5-FAM (pH 9.0) | 491.9 | 518.1 | 503.5 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| 5-ROX (pH 7.0) | 578.2 | 604 | 589.7 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 600 | 3 | 80 | 60 | ||||
| 5-TAMRA (pH 7.0) | 553 | 576 | 563.4 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 580 | 3 | 10 | 45 | ||||
| 6-carboxyrhodamine 6G (pH 7.0) | 525 | 547 | 536 | Excitation | 520 | 6 | 35 | 90 |
| Emission | 546 | 3 | 10 | 45 | ||||
| 6-JOE (pH 9.0) | 519.8 | 548 | 532.7 | Excitation | 520 | 6 | 35 | 90 |
| Emission | 550 | 3 | 80 | 60 | ||||
| 7-amino-4-methylcoumarin (pH 7.0) | 346 | 442 | 396.5 | Excitation | 340 | 6 | 26 | 75 |
| Emission | 442 | 3 | 10 | 40 | ||||
| 7-aminoactinomycin D (DNA) | 548.1 | 647.9 | 601.8 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 647 | 3 | 10 | 45 | ||||
| 7-hydroxy-4-methylcoumarin (pH 9.0) | 360.1 | 447.9 | 403 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 447 | 6 | 60 | 90 | ||||
| Acridine orange (DNA) | 502.1 | 526 | 513 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 531 | 6 | 40 | 90 | ||||
| Alexa Fluor 350 goat anti-mouse IgG antibody (pH 8.0) | 343 | 441 | 392.9 | Excitation | 340 | 6 | 26 | 75 |
| Emission | 442 | 3 | 10 | 40 | ||||
| Alexa Fluor 405 goat anti-mouse IgG antibody (pH 7.2) | 401.1 | 422.1 | 409 | Excitation | 400 | 3 | 50 | 55 |
| Emission | 420 | 3 | 10 | 40 | ||||
| Alexa Fluor 430 goat anti-mouse IgG antibody (pH 7.2) | 431.2 | 540 | 492.9 | Excitation | 430 | 3 | 10 | 40 |
| Emission | 540 | 3 | 10 | 45 | ||||
| Alexa Fluor 488 dye-labeled oligonucleotide (H2O) | 493.1 | 519 | 505.8 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| Alexa Fluor 488 goat anti-mouse IgG antibody (pH 8.0) | 498.9 | 520 | 508.8 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| Alexa Fluor 488 hydrazide (H2O) | 492.8 | 517.1 | 504.9 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| Alexa Fluor 514 goat anti-mouse IgG antibody (pH 8.0) | 518 | 542.9 | 529.8 | Excitation | 520 | 6 | 35 | 90 |
| Emission | 543 | 6 | 22 | 90 | ||||
| Alexa Fluor 532 goat anti-mouse IgG antibody (pH 7.2) | 533.9 | 553 | 543 | Excitation | 535 | 3 | 10 | 45 |
| Emission | 550 | 3 | 80 | 60 | ||||
| Alexa Fluor 546 dye-labeled oligonucleotide (H2O) | 554.1 | 574 | 563.8 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Alexa Fluor 546 goat anti-mouse IgG antibody (pH 7.2) | 561.3 | 572 | 566.9 | Excitation | 562 | 6 | 40 | 90 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Alexa Fluor 555 dye-labeled oligonucleotide (H2O) | 552.3 | 567.1 | 559.6 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Alexa Fluor 555 goat anti-mouse IgG antibody (pH 7.2) | 553.2 | 567.9 | 560.2 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Alexa Fluor 555 hydrazide (H2O) | 551.1 | 564 | 557.1 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 562 | 6 | 40 | 90 | ||||
| Alexa Fluor 568 goat anti-mouse IgG antibody (pH 7.2) | 578.9 | 603 | 590.2 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 600 | 3 | 80 | 60 | ||||
| Alexa Fluor 568 hydrazide (H2O) | 572.9 | 599.9 | 585.8 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 600 | 3 | 80 | 60 | ||||
| Alexa Fluor 594 cadaverine (H2O) | 587.9 | 614.1 | 600.1 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| Alexa Fluor 594 dye-labeled oligonucleotide (H2O) | 590.9 | 621 | 605.7 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 620 | 3 | 10 | 45 | ||||
| Alexa Fluor 594 goat anti-mouse IgG antibody (pH 7.2) | 590.2 | 618.1 | 604.2 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 620 | 3 | 10 | 45 | ||||
| Alexa Fluor 610 goat anti-mouse IgG antibody (pH 7.2) | 611.9 | 630 | 620.2 | Excitation | 610 | 3 | 10 | 45 |
| Emission | 628 | 6 | 40 | 90 | ||||
| Alexa Fluor 610-R-phycoerythrin streptavidin (pH 7.2) | 566.9 | 627 | 589.7 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 628 | 6 | 40 | 90 | ||||
| Alexa Fluor 633 goat anti-mouse IgG antibody (pH 7.2) | 631.1 | 649.9 | 640.2 | Excitation | 632 | 4 | 10 | 85 |
| Emission | 650 | 4 | 10 | 85 | ||||
| Alexa Fluor 635 goat anti-mouse IgG antibody (pH 7.2) | 633.1 | 647.9 | 639.9 | Excitation | 632 | 4 | 10 | 85 |
| Emission | 647 | 3 | 10 | 45 | ||||
| Alexa Fluor 647 dye-labeled oligonucleotide (H2O) | 650.1 | 667.9 | 659.1 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 671 | 4 | 10 | 85 | ||||
| Alexa Fluor 647 goat anti-mouse IgG antibody (pH 7.2) | 649.9 | 671 | 660.6 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 671 | 4 | 10 | 85 | ||||
| Alexa Fluor 647 hydrazide (H2O) | 649.1 | 670 | 659.2 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 671 | 4 | 10 | 85 | ||||
| Alexa Fluor 647-R-phycoerythrin streptavidin (pH 7.2) | 568.2 | 666 | 648.9 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 671 | 4 | 10 | 85 | ||||
| Alexa Fluor 660 goat anti-mouse IgG antibody (pH 7.2) | 663.1 | 690.9 | 679.3 | Excitation | 656 | 4 | 10 | 85 |
| Emission | 690 | 3 | 10 | 50 | ||||
| Alexa Fluor 680 goat anti-mouse IgG antibody (pH 7.2) | 680.9 | 703.9 | 692.8 | Excitation | 676 | 3 | 10 | 50 |
| Emission | 700 | 3 | 80 | 60 | ||||
| Alexa Fluor 680-R-phycoerythrin streptavidin (pH 7.2) | 569.6 | 702 | 687.1 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 700 | 3 | 80 | 60 | ||||
| Alexa Fluor 700 goat anti-mouse IgG antibody (pH 7.2) | 695.9 | 719 | 708.3 | Excitation | 694 | 3 | 10 | 50 |
| Emission | 730 | 3 | 10 | 50 | ||||
| Alexa Fluor 750 goat anti-mouse IgG antibody (pH 7.2) | 752.4 | 776.1 | 763.6 | Excitation | 766 | 3 | 10 | 50 |
| Emission | 780 | 3 | 10 | 50 | ||||
| Alexa Fluor 790 goat anti-mouse IgG antibody (pH 7.2) | 781.8 | 804.9 | 791.1 | Excitation | 780 | 3 | 10 | 50 |
| Emission | 800 | 3 | 10 | 50 | ||||
| Alexa Fluorr 350 | 346.9 | 443.9 | 394.2 | Excitation | 340 | 6 | 26 | 75 |
| Emission | 442 | 3 | 10 | 40 | ||||
| Allophycocyanin (pH 7.5) | 651 | 660.1 | 655.6 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 656 | 4 | 10 | 85 | ||||
| Amplex UltraRed peroxidation product (pH 7.5) | 567.9 | 581 | 575.2 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 580 | 3 | 10 | 45 | ||||
| BOBO-1 (DNA) | 461.2 | 483.9 | 472.6 | Excitation | 458 | 3 | 10 | 45 |
| Emission | 483 | 6 | 32 | 90 | ||||
| BOBO-3 (DNA) | 570.2 | 605.1 | 590.2 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| BODIPY 630-650-X (MeOH) | 625.2 | 641 | 633.7 | Excitation | 624 | 6 | 40 | 90 |
| Emission | 640 | 3 | 10 | 45 | ||||
| BODIPY 650-665-X (MeOH) | 645.9 | 664.1 | 654.9 | Excitation | 647 | 3 | 10 | 45 |
| Emission | 671 | 4 | 10 | 85 | ||||
| BODIPY FL (MeOH) | 502.1 | 511.1 | 506.7 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 510 | 6 | 84 | 90 | ||||
| BODIPY FL goat anti-mouse IgG antibody (pH 7.2) | 505.3 | 512 | 504.1 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 510 | 6 | 84 | 90 | ||||
| BODIPY R6G (MeOH) | 528.2 | 546.9 | 539.2 | Excitation | 531 | 6 | 40 | 90 |
| Emission | 546 | 3 | 10 | 45 | ||||
| BODIPY TMR-X (MeOH) | 544 | 570.1 | 559.3 | Excitation | 543 | 6 | 22 | 90 |
| Emission | 568 | 3 | 10 | 45 | ||||
| BODIPY TMR-X goat anti-mouse IgG antibody (pH 7.2) | 544 | 573 | 561.4 | Excitation | 543 | 6 | 22 | 90 |
| Emission | 568 | 3 | 10 | 45 | ||||
| BODIPY TR-X (MeOH) | 588 | 621 | 607.2 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 620 | 3 | 10 | 45 | ||||
| BODIPY TR-X phallacidin (pH 7.0) | 590 | 621 | 608.1 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 620 | 3 | 10 | 45 | ||||
| Calcein (pH 9.0) | 493.9 | 514 | 503.6 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| Calcium Crimson (Ca2+) | 589 | 608.9 | 598.3 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| Calcium Green-1 (Ca2+) | 507 | 529.1 | 517 | Excitation | 508 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| Calcium Orange (Ca2+) | 549.1 | 574 | 561.3 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Carboxynaphthofluorescein (pH 10.0) | 598.9 | 674 | 637 | Excitation | 600 | 3 | 80 | 60 |
| Emission | 676 | 3 | 10 | 50 | ||||
| Cascade Blue BSA (pH 7.0) | 401.1 | 419 | 407.8 | Excitation | 400 | 3 | 50 | 55 |
| Emission | 420 | 3 | 10 | 40 | ||||
| Cascade Yellow goat anti-mouse IgG antibody (pH 8.0) | 399.1 | 549.1 | 472.4 | Excitation | 400 | 3 | 50 | 55 |
| Emission | 550 | 3 | 80 | 60 | ||||
| CellMask Deep Red plasma membrane stain | 659 | 675.9 | 668.6 | Excitation | 656 | 4 | 10 | 85 |
| Emission | 676 | 3 | 10 | 50 | ||||
| CellMask Orange plasma membrane stain | 556 | 571.9 | 563.5 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 568 | 3 | 10 | 45 | ||||
| CellTrace BODIPY TR methyl ester (lipid) | 597.9 | 624.9 | 613.5 | Excitation | 600 | 3 | 80 | 60 |
| Emission | 624 | 6 | 40 | 90 | ||||
| CellTrace calcein violet (pH 7.2) | 400.1 | 452.1 | 427.7 | Excitation | 400 | 3 | 50 | 55 |
| Emission | 450 | 3 | 80 | 60 | ||||
| CellTracker Red CMTPX | 586 | 614 | 597.1 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| CellTracker Violet BMQC+GSH | 404.1 | 525.9 | 470.2 | Excitation | 405 | 4 | 10 | 85 |
| Emission | 531 | 6 | 40 | 90 | ||||
| CFP (pH 7.4) | 433.1 | 475.1 | 465.3 | Excitation | 436 | 3 | 10 | 40 |
| Emission | 472 | 6 | 30 | 90 | ||||
| CMR (pH 7.4) | 547 | 574.9 | 560.5 | Excitation | 546 | 3 | 10 | 45 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Cy3 dye-labeled IgG antibody (pH 7.2) | 553.9 | 565.8 | 559.5 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Cy5 dye-labeled IgG antibody (pH 7.2) | 648.9 | 666.1 | 657.9 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 671 | 4 | 10 | 85 | ||||
| CyQUA GR (DNA) | 502.1 | 522.9 | 512.2 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| DAF-FM (NO) | 495 | 519 | 505.4 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| DAPI (DNA) | 359 | 460.9 | 401.1 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 458 | 3 | 10 | 45 | ||||
| Dapoxyl (2-aminoethyl)sulfonamide (MeOH) | 373.1 | 578.9 | 459.2 | Excitation | 377 | 6 | 50 | 85 |
| Emission | 580 | 3 | 10 | 45 | ||||
| DDAO (pH 9.0) | 647.7 | 657.1 | 653.4 | Excitation | 647 | 3 | 10 | 45 |
| Emission | 656 | 4 | 10 | 85 | ||||
| Di-8-ANEPPS (lipid) | 467 | 631 | 536.9 | Excitation | 467 | 3 | 10 | 45 |
| Emission | 632 | 4 | 10 | 85 | ||||
| DiA (lipid) | 456.9 | 585.9 | 521.5 | Excitation | 458 | 3 | 10 | 45 |
| Emission | 589 | 3 | 10 | 45 | ||||
| DiD (lipid) | 647.9 | 669.9 | 658.3 | Excitation | 647 | 3 | 10 | 45 |
| Emission | 671 | 4 | 10 | 85 | ||||
| DiFMU (pH 3.0) | 319.9 | 451.1 | 370.4 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 450 | 3 | 80 | 60 | ||||
| DiFMU (pH 9.0) | 358 | 450 | 406.5 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 450 | 3 | 80 | 60 | ||||
| DiI (lipid) | 550.9 | 568.9 | 559.6 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 568 | 3 | 10 | 45 | ||||
| DiO (lipid) | 489.2 | 506 | 497.8 | Excitation | 488 | 3 | 10 | 45 |
| Emission | 505 | 3 | 10 | 45 | ||||
| DiR (lipid) | 749.9 | 782 | 764.7 | Excitation | 766 | 3 | 10 | 50 |
| Emission | 780 | 3 | 10 | 50 | ||||
| EGFP (pH 7) | 488.9 | 508.1 | 500.1 | Excitation | 488 | 3 | 10 | 45 |
| Emission | 508 | 3 | 10 | 45 | ||||
| ELF 97 alcohol (pH 8.0) | 300 | 536 | 405.2 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 536 | 6 | 40 | 90 | ||||
| Eosin goat anti-mouse IgG antibody (pH 8.0) | 525 | 545.9 | 535.5 | Excitation | 520 | 6 | 35 | 90 |
| Emission | 546 | 3 | 10 | 45 | ||||
| ER-Tracker Blue-White DPX (lipid) | 372.1 | 556.8 | 422.2 | Excitation | 377 | 6 | 50 | 85 |
| Emission | 562 | 6 | 40 | 90 | ||||
| Ethidium bromide (DNA) | 300 | 603 | 550.2 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 600 | 3 | 80 | 60 | ||||
| Ethidium homodimer-1 (DNA) | 527 | 616.9 | 574.3 | Excitation | 531 | 6 | 40 | 90 |
| Emission | 620 | 3 | 10 | 45 | ||||
| FITC goat anti-mouse IgG antibody (pH 8.0) | 495.1 | 519 | 506.5 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| FlAsH-CCPGCC | 511 | 530.1 | 520.1 | Excitation | 510 | 6 | 84 | 90 |
| Emission | 531 | 6 | 40 | 90 | ||||
| Fluo-3 (Ca2+) | 506 | 527 | 516.1 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| Fluo-4 (Ca2+) | 494 | 515.9 | 504.3 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| Fluorescein (pH 13.0) | 492.9 | 513 | 502.1 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| Fluorescein (pH 9.0) | 489.9 | 513.2 | 501.1 | Excitation | 488 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| Fluorescein dextran (pH 8.0) | 501.1 | 524 | 511.8 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| Fluorescein goat anti-mouse IgG antibody (pH 8.0) | 497.9 | 517 | 507.1 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 515 | 3 | 10 | 45 | ||||
| FluoSpheres blue fluorescent microspheres (H2O) | 355.9 | 414 | 383.8 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 415 | 3 | 10 | 40 | ||||
| FluoSpheres crimson fluorescent microspheres (H20) | 619.9 | 646.1 | 635.3 | Excitation | 620 | 3 | 10 | 45 |
| Emission | 647 | 3 | 10 | 45 | ||||
| FluoSpheres dark red fluorescent microspheres (H2O) | 656 | 683.1 | 671.4 | Excitation | 656 | 4 | 10 | 85 |
| Emission | 690 | 3 | 10 | 50 | ||||
| FluoSpheres orange fluorescent microspheres (H2O) | 540 | 559.1 | 551.3 | Excitation | 540 | 3 | 10 | 45 |
| Emission | 562 | 6 | 40 | 90 | ||||
| FluoSpheres red fluorescent microspheres (H2O) | 576.1 | 607 | 594.5 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| FluoSpheres yellow-green fluorescent microspheres (H2O) | 503.1 | 513.9 | 508.1 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| FluxOR thallium indicator (pH 7.2) | 492.9 | 521 | 505.2 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| FM 1-43 (lipid) | 473 | 579 | 522.9 | Excitation | 472 | 6 | 30 | 90 |
| Emission | 580 | 3 | 10 | 45 | ||||
| FM 4-64 (2% CHAPS) | 505 | 725.1 | 621.1 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 730 | 3 | 10 | 50 | ||||
| FxCycle Far Red stain | 640.2 | 658 | 650.1 | Excitation | 640 | 3 | 10 | 45 |
| Emission | 656 | 4 | 10 | 85 | ||||
| FxCycle Violet stain | 359 | 460.9 | 401.1 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 458 | 3 | 10 | 45 | ||||
| HCS CellMask Blue stain | 346.9 | 443.9 | 394.2 | Excitation | 340 | 6 | 26 | 75 |
| Emission | 442 | 3 | 10 | 40 | ||||
| HCS CellMask Deep Red stain | 649.1 | 670 | 659.2 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 671 | 4 | 10 | 85 | ||||
| HCS CellMask Green Stain | 492.8 | 517.1 | 504.9 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 515 | 3 | 10 | 45 | ||||
| HCS CellMask Orange stain | 551.1 | 564 | 557.1 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 562 | 6 | 40 | 90 | ||||
| HCS CellMask Red cytoplasmic (nuclear stain) | 621.9 | 644 | 632.6 | Excitation | 620 | 3 | 10 | 45 |
| Emission | 647 | 3 | 10 | 45 | ||||
| HCS CellMask Red stain | 587.9 | 614.1 | 600.1 | Excitation | 589 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| HCS LipidTOX Deep Red neutral lipid stain (triglyceride) | 634.9 | 652 | 644.1 | Excitation | 636 | 4 | 10 | 85 |
| Emission | 650 | 4 | 10 | 85 | ||||
| HCS LipidTOX Green neutral lipid stain (triglyceride) | 498 | 507 | 502.2 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 508 | 3 | 10 | 45 | ||||
| HCS LipidTOX Green phospholipidosis detection reagent | 504 | 536 | 518.2 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 536 | 6 | 40 | 90 | ||||
| HCS LipidTOX Red neutral lipid stain (triglyceride) | 581.9 | 615.9 | 601.8 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 620 | 3 | 10 | 45 | ||||
| HCS LipidTOX Red phospholipidosis detection reagent | 584.2 | 608 | 596.3 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| HCS NuclearMask Deep Red stain (DNA) | 637.9 | 686.1 | 663.1 | Excitation | 636 | 4 | 10 | 85 |
| Emission | 690 | 3 | 10 | 50 | ||||
| HCS NuclearMask Red stain | 621.9 | 644 | 632.6 | Excitation | 620 | 3 | 10 | 45 |
| Emission | 647 | 3 | 10 | 45 | ||||
| Hoechst 33258 (DNA) | 352 | 454.9 | 394.9 | Excitation | 340 | 6 | 26 | 75 |
| Emission | 455 | 3 | 10 | 45 | ||||
| JOJO-1 (DNA) | 530 | 544 | 536.2 | Excitation | 531 | 6 | 40 | 90 |
| Emission | 543 | 6 | 22 | 90 | ||||
| LDS 751 (DNA) | 560.1 | 711.1 | 649 | Excitation | 562 | 6 | 40 | 90 |
| Emission | 700 | 3 | 80 | 60 | ||||
| LIVE DEAD Fixable Aqua Dead Cell Stain (pH 7.2) | 367 | 526.1 | 440 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 531 | 6 | 40 | 90 | ||||
| LIVE DEAD Fixable Blue Dead Cell Stain | 343 | 441 | 392.9 | Excitation | 340 | 6 | 26 | 75 |
| Emission | 442 | 3 | 10 | 40 | ||||
| LIVE DEAD Fixable Far Red Dead Cell Stain | 649.9 | 671 | 660.6 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 671 | 4 | 10 | 85 | ||||
| LIVE DEAD Fixable Green Dead Cell Stain | 497.9 | 525.9 | 510.1 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 531 | 6 | 40 | 90 | ||||
| LIVE DEAD Fixable Near-IR Dead Cell Stain | 752.4 | 776.1 | 763.6 | Excitation | 766 | 3 | 10 | 50 |
| Emission | 780 | 3 | 10 | 50 | ||||
| LIVE DEAD Fixable Red Dead Cell Stain | 595 | 613 | 603.9 | Excitation | 593 | 6 | 40 | 90 |
| Emission | 610 | 3 | 10 | 45 | ||||
| LIVE DEAD Fixable Violet Dead Cell Stain | 403.9 | 454.9 | 431.4 | Excitation | 405 | 4 | 10 | 85 |
| Emission | 455 | 3 | 10 | 45 | ||||
| LIVE DEAD Fixable Yellow Dead Cell Stain (pH 7.2) | 400.1 | 550.9 | 469.4 | Excitation | 400 | 3 | 50 | 55 |
| Emission | 550 | 3 | 80 | 60 | ||||
| LOLO-1 (DNA) | 568.1 | 580.1 | 574.4 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Lucifer yellow CH (H2O) | 427.9 | 544 | 482.7 | Excitation | 430 | 3 | 10 | 40 |
| Emission | 543 | 6 | 22 | 90 | ||||
| LysoSensor Blue (pH 5.0) | 395 | 424 | 397.6 | Excitation | 394 | 3 | 10 | 30 |
| Emission | 420 | 3 | 10 | 40 | ||||
| LysoSensor Green (pH 5.0) | 446.8 | 502 | 483.2 | Excitation | 447 | 6 | 60 | 90 |
| Emission | 500 | 6 | 24 | 90 | ||||
| LysoTracker Blue (MeOH) | 373 | 421.8 | 396.4 | Excitation | 377 | 6 | 50 | 85 |
| Emission | 420 | 3 | 10 | 40 | ||||
| LysoTracker Green (pH 5.2) | 500.9 | 509.1 | 505.2 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 510 | 6 | 84 | 90 | ||||
| LysoTracker Red (pH 5.2) | 573.1 | 592 | 582.6 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 593 | 6 | 40 | 90 | ||||
| LysoTracker Yellow HCK-123 | 488 | 565 | 509 | Excitation | 488 | 3 | 10 | 45 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Magnesium Green (Mg2+) | 507 | 531 | 517.2 | Excitation | 508 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| Marina Blue goat anti-mouse IgG antibody (pH 8.0) | 363.8 | 461.1 | 412 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 458 | 3 | 10 | 45 | ||||
| mBBr+GSH (pH 8.0) | 394 | 490.1 | 449.2 | Excitation | 394 | 3 | 10 | 30 |
| Emission | 492 | 3 | 10 | 45 | ||||
| MitoTracker Deep Red FM (MeOH) | 641 | 661.9 | 652.2 | Excitation | 640 | 3 | 10 | 45 |
| Emission | 656 | 4 | 10 | 85 | ||||
| MitoTracker Green FM (MeOH) | 490.1 | 512 | 502 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 510 | 6 | 84 | 90 | ||||
| MitoTracker Orange (MeOH) | 551.2 | 575 | 562.1 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 580 | 3 | 10 | 45 | ||||
| MitoTracker Red (MeOH) | 578.2 | 598.1 | 587.3 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 600 | 3 | 80 | 60 | ||||
| mRFP (pH 7.4) | 554.8 | 583.2 | 565.1 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 580 | 3 | 10 | 45 | ||||
| NBD-X (MeOH) | 467 | 537.9 | 499.2 | Excitation | 467 | 3 | 10 | 45 |
| Emission | 536 | 6 | 40 | 90 | ||||
| NeuroTrace 500-525 green fluorescent Nissl stain (RNA) | 497 | 524 | 508.2 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| Nile blue (EtOH) | 630.6 | 660 | 649 | Excitation | 632 | 4 | 10 | 85 |
| Emission | 656 | 4 | 10 | 85 | ||||
| Nile red (phospholipid) | 553 | 636.9 | 602.5 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 636 | 4 | 10 | 85 | ||||
| Nile red (triglyceride) | 510.1 | 583.1 | 556 | Excitation | 510 | 6 | 84 | 90 |
| Emission | 580 | 3 | 10 | 45 | ||||
| OFP | 546.9 | 567 | 556.8 | Excitation | 546 | 3 | 10 | 45 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Oregon Green 488 DHPE | 504 | 536 | 518.2 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 536 | 6 | 40 | 90 | ||||
| Oregon Green 488 goat anti-mouse IgG antibody (pH 8.0) | 497.9 | 525.9 | 510.1 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 531 | 6 | 40 | 90 | ||||
| Oregon Green 514 goat anti-mouse IgG antibody (pH 8.0) | 512.8 | 533 | 521.6 | Excitation | 515 | 3 | 10 | 45 |
| Emission | 532 | 4 | 10 | 85 | ||||
| Pacific Blue dye-labeled oligonucleotide (H2O) | 402 | 454.9 | 430.5 | Excitation | 400 | 3 | 50 | 55 |
| Emission | 455 | 3 | 10 | 45 | ||||
| Pacific Blue goat anti-mouse IgG antibody (pH 8.0) | 403.9 | 454.9 | 431.4 | Excitation | 405 | 4 | 10 | 85 |
| Emission | 455 | 3 | 10 | 45 | ||||
| Pacific Orange goat anti-mouse IgG antibody (pH 7.2) | 400.1 | 550.9 | 469.4 | Excitation | 400 | 3 | 50 | 55 |
| Emission | 550 | 3 | 80 | 60 | ||||
| pHrodo, succinimidyl ester | 560 | 586.9 | 572.4 | Excitation | 562 | 6 | 40 | 90 |
| Emission | 589 | 3 | 10 | 45 | ||||
| PicoGreen dsDNA quantitation reagent | 501.9 | 522.1 | 511.9 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| POPO-1 (DNA) | 433.1 | 457.1 | 446.1 | Excitation | 436 | 3 | 10 | 40 |
| Emission | 458 | 3 | 10 | 45 | ||||
| POPO-3 (DNA) | 533 | 573.9 | 556.4 | Excitation | 532 | 4 | 10 | 85 |
| Emission | 568 | 3 | 10 | 45 | ||||
| PO-PRO-1 (DNA) | 434.1 | 456.8 | 445.8 | Excitation | 436 | 3 | 10 | 40 |
| Emission | 458 | 3 | 10 | 45 | ||||
| PO-PRO-3 (DNA) | 538.9 | 571 | 556.1 | Excitation | 540 | 3 | 10 | 45 |
| Emission | 568 | 3 | 10 | 45 | ||||
| Propidium iodide (DNA) | 304.9 | 617.1 | 565.7 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 620 | 3 | 10 | 45 | ||||
| Pro-Q Diamond phosphoprotein gel stain | 556.2 | 582.9 | 569 | Excitation | 562 | 6 | 40 | 90 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Pro-Q Emerald 300 reagent | 300 | 530.1 | 402.9 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 531 | 6 | 40 | 90 | ||||
| QDot 525 streptavidin (pH 7.2) | 300 | 525.2 | 628.9 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 520 | 6 | 35 | 90 | ||||
| QDot 545 streptavidin (pH 7.2) | 300 | 543 | 515.2 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 543 | 6 | 22 | 90 | ||||
| QDot 565 streptavidin (pH 7.2) | 300 | 564 | 648 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 562 | 6 | 40 | 90 | ||||
| Qdot 585 streptavidin (pH 7.2) | 300 | 587.9 | 694.9 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 589 | 3 | 10 | 45 | ||||
| Qdot 605 streptavidin (pH 7.2) | 300 | 603.1 | 580 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 600 | 3 | 80 | 60 | ||||
| Qdot 625 streptavidin (pH 7.2) | 300 | 621 | 586 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 620 | 3 | 10 | 45 | ||||
| Qdot 655 streptavidin (pH 7.2) | 300 | 654.1 | 619.3 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 656 | 4 | 10 | 85 | ||||
| Qdot 705 streptavidin (pH 7.2) | 300 | 701.8 | 638 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 700 | 3 | 80 | 60 | ||||
| Qdot 800 streptavidin (pH 7.2) | 300 | 791.9 | 685.5 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 800 | 3 | 10 | 50 | ||||
| Quinine sulfate (0.5 M H2SO4) | 348.9 | 461 | 392.5 | Excitation | 340 | 6 | 26 | 75 |
| Emission | 458 | 3 | 10 | 45 | ||||
| ReAsH-CCPGCC | 592.2 | 606 | 600.1 | Excitation | 593 | 6 | 40 | 90 |
| Emission | 610 | 3 | 10 | 45 | ||||
| Resorufin (pH 9.0) | 571.2 | 584 | 577.7 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Rhod-2 (Ca2+) | 553 | 577 | 563.8 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Rhodamine 110 (pH 7) | 497 | 519.9 | 508.1 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| Rhodamine 123 (MeOH) | 507.2 | 528.9 | 517.1 | Excitation | 508 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| Rhodamine Green (pH 7.0) | 497 | 523 | 510 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| Rhodamine phalloidin (pH 7.0) | 558.2 | 575 | 566.9 | Excitation | 562 | 6 | 40 | 90 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Rhodamine Red-X goat anti-mouse IgG antibody (pH 8.0) | 572.9 | 591 | 581.7 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 589 | 3 | 10 | 45 | ||||
| R-phycoerythrin (pH 7.5) | 565.2 | 576 | 570.6 | Excitation | 568 | 3 | 10 | 45 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Sodium Green (Na+) | 507.2 | 532 | 518.3 | Excitation | 508 | 3 | 10 | 45 |
| Emission | 532 | 4 | 10 | 85 | ||||
| Sulforhodamine 101 (EtOH) | 578.2 | 593.1 | 585.3 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 593 | 6 | 40 | 90 | ||||
| SYBR Gold nucleic acid gel stain (DNA) | 496 | 539 | 515 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 540 | 3 | 10 | 45 | ||||
| SYBR Green I nucleic acid gel stain (DNA) | 497.9 | 521.9 | 508.9 | Excitation | 500 | 6 | 24 | 90 |
| Emission | 520 | 6 | 35 | 90 | ||||
| SYBR Safe DNA gel stain (DNA) | 509 | 525.9 | 516.5 | Excitation | 510 | 6 | 84 | 90 |
| Emission | 531 | 6 | 40 | 90 | ||||
| SYPRO Orange protein gel stain (BSA) | 490.9 | 585.9 | 538.4 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 589 | 3 | 10 | 45 | ||||
| SYPRO Ruby protein gel stain | 300 | 631.1 | 563.8 | Excitation | 334 | 3 | 10 | 25 |
| Emission | 632 | 4 | 10 | 85 | ||||
| SYTO 11 (DNA) | 506.9 | 525 | 514.9 | Excitation | 508 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| SYTO 13 (DNA) | 488 | 505.9 | 496.2 | Excitation | 488 | 3 | 10 | 45 |
| Emission | 505 | 3 | 10 | 45 | ||||
| SYTO 16 (DNA) | 489 | 519.9 | 503.1 | Excitation | 488 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| SYTO 17 (DNA) | 619.1 | 638 | 628.3 | Excitation | 620 | 3 | 10 | 45 |
| Emission | 636 | 4 | 10 | 85 | ||||
| SYTO 45 (DNA) | 452 | 485 | 467 | Excitation | 450 | 3 | 80 | 60 |
| Emission | 486 | 3 | 10 | 45 | ||||
| SYTO 59 (DNA) | 621.9 | 644 | 632.6 | Excitation | 620 | 3 | 10 | 45 |
| Emission | 647 | 3 | 10 | 45 | ||||
| SYTO 60 (DNA) | 649.8 | 680.9 | 665.4 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 676 | 3 | 10 | 50 | ||||
| SYTO 61 (DNA) | 618.1 | 652.9 | 634.2 | Excitation | 620 | 3 | 10 | 45 |
| Emission | 650 | 4 | 10 | 85 | ||||
| SYTO 62 (DNA) | 649.8 | 680.9 | 665.4 | Excitation | 650 | 4 | 10 | 85 |
| Emission | 676 | 3 | 10 | 50 | ||||
| SYTO 82 (DNA) | 540 | 559.1 | 549.2 | Excitation | 540 | 3 | 10 | 45 |
| Emission | 562 | 6 | 40 | 90 | ||||
| SYTO 9 (DNA) | 482.9 | 500.1 | 490.9 | Excitation | 483 | 6 | 32 | 90 |
| Emission | 500 | 6 | 24 | 90 | ||||
| SYTO RNA Select green fluorescent cell stain (RNA) | 503 | 527 | 514.8 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| SYTOX AADvanced dead cell stain (DNA) | 546.1 | 647 | 599.6 | Excitation | 546 | 3 | 10 | 45 |
| Emission | 647 | 3 | 10 | 45 | ||||
| SYTOX Blue (DNA) | 445 | 470.1 | 457.4 | Excitation | 447 | 6 | 60 | 90 |
| Emission | 470 | 3 | 10 | 45 | ||||
| SYTOX Green (DNA) | 503.9 | 524 | 513.6 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 520 | 6 | 35 | 90 | ||||
| SYTOX Orange (DNA) | 547 | 570 | 558.3 | Excitation | 546 | 3 | 10 | 45 |
| Emission | 568 | 3 | 10 | 45 | ||||
| SYTOX Red (DNA) | 640.2 | 658 | 650.1 | Excitation | 640 | 3 | 10 | 45 |
| Emission | 656 | 4 | 10 | 85 | ||||
| Tetramethylrhodamine dextran (pH 7.0) | 554.1 | 582 | 567.6 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Tetramethylrhodamine goat anti-mouse IgG antibody (pH 8.0) | 552 | 578 | 564.1 | Excitation | 550 | 3 | 80 | 60 |
| Emission | 580 | 3 | 10 | 45 | ||||
| Texas Red dextran (H2O) | 592 | 613.9 | 601.8 | Excitation | 593 | 6 | 40 | 90 |
| Emission | 610 | 3 | 10 | 45 | ||||
| Texas Red DHPE | 584.2 | 608 | 596.3 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 610 | 3 | 10 | 45 | ||||
| Texas Red-X goat anti-mouse IgG antibody (pH 7.2 ) | 595 | 613 | 603.9 | Excitation | 593 | 6 | 40 | 90 |
| Emission | 610 | 3 | 10 | 45 | ||||
| ThiolTracker Violet+GSH | 404.1 | 525.9 | 470.2 | Excitation | 405 | 4 | 10 | 85 |
| Emission | 531 | 6 | 40 | 90 | ||||
| TO-PRO-1 (DNA) | 515 | 531 | 522.4 | Excitation | 515 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| TO-PRO-3 (DNA) | 641.9 | 657 | 649.5 | Excitation | 640 | 3 | 10 | 45 |
| Emission | 656 | 4 | 10 | 85 | ||||
| TOTO-1 (DNA) | 513.9 | 530.9 | 522.3 | Excitation | 515 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| TOTO-3 (DNA) | 641.9 | 661 | 651.8 | Excitation | 640 | 3 | 10 | 45 |
| Emission | 656 | 4 | 10 | 85 | ||||
| Vybrant DyeCycle Green stain (DNA) | 506 | 533.9 | 518.5 | Excitation | 505 | 3 | 10 | 45 |
| Emission | 535 | 3 | 10 | 45 | ||||
| Vybrant DyeCycle Orange stain (DNA) | 519 | 563.1 | 531.9 | Excitation | 520 | 6 | 35 | 90 |
| Emission | 562 | 6 | 40 | 90 | ||||
| Vybrant DyeCycle Ruby stain (DNA) | 636.9 | 685.2 | 662.9 | Excitation | 636 | 4 | 10 | 85 |
| Emission | 690 | 3 | 10 | 50 | ||||
| Vybrant DyeCycle Violet stain (DNA) | 369.1 | 436.9 | 401.5 | Excitation | 365 | 3 | 10 | 25 |
| Emission | 436 | 3 | 10 | 40 | ||||
| X-rhod-1 (Ca2+) | 580 | 601.1 | 589.7 | Excitation | 580 | 3 | 10 | 45 |
| Emission | 600 | 3 | 80 | 60 | ||||
| YFP (pH 7.4) | 513.1 | 530 | 521.1 | Excitation | 515 | 3 | 10 | 45 |
| Emission | 531 | 6 | 40 | 90 | ||||
| YO-PRO-1 (DNA) | 491.1 | 506.1 | 499.2 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 505 | 3 | 10 | 45 | ||||
| YO-PRO-3 (DNA) | 612.9 | 628.9 | 620.3 | Excitation | 610 | 3 | 10 | 45 |
| Emission | 628 | 6 | 40 | 90 | ||||
| YOYO-1 (DNA) | 490.9 | 508 | 499.3 | Excitation | 492 | 3 | 10 | 45 |
| Emission | 508 | 3 | 10 | 45 | ||||
| YOYO-3 (DNA) | 612.2 | 631.1 | 622.5 | Excitation | 610 | 3 | 10 | 45 |
| Emission | 632 | 4 | 10 | 85 |
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